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Interactions between Viral and Motor Proteins - a Gene Therapy Vector for Spinal Cord Disease? Retrograde Cellular Transport of Herpes Simplex Virus.

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180 страниц
2008 год
Издательство:   Книга по Требованию
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2781 руб
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Описание:To study retrograde transport of HSV-1, capsid and tegument proteins were tested for interactions with the motor cytoplasmic dynein, using a yeast two-hybrid system and in vitro pull down assays. A strong interaction was demonstrated between the HSV-1 outer capsid protein VP26 (UL35) and the homologous 14 kDa dynein light chains RP3 and Tctex1. Herpes simplex virus type 1 (HSV-1) establishes life-long latent infection in sensory neurones, making it an ideal gene therapy vector to target neuronal cells. Capsids containing VP26 moved closer to the cell nucleus, while capsids without VP26 remained in a random distribution. The functional importance of VP26 for retrograde transport was confirmed by microinjecting recombinant HSV-1 capsids into living cells. It is hoped that these findings will help in the development of a synthetic viral vector, which may allow targeted gene therapy in patients with neurological diseases. Our results suggest that the HSV-1 outer capsid protein VP26 mediates binding of incoming capsids to the retrograde motor cytoplasmic dynein during cellular infection, through interactions with dynein light chains.


Orthomol Immun Junior Сила иммунитета на страже Вашего ребенка Orthomol Immun Junior Сила иммунитета на страже Вашего ребенка

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The Remembered Present: A Biological Theory of Consciousness. Basic Books, New York, 1989. . Bright Air, Brilliant Fire. Basic Books, New York, 1992. Ehrenfeld, David. "A Techno-Pox Upon the Land." Harper's Magazine, October 1997. Ellul, Jacques. The Technological Society. Knopf, New York, 1964. Faux, Jeff, and Larry Mishel. "Inequality and the Global Economy," in Hutton and Giddens (2000). Fischer, Aline, Thomas Oberholzer, and Pier Luigi Luisi. "Giant vesicles as models to study the interactions between membranes and proteins." Biochimica et Biophyska Ada, vol. 1467, pp. 177-88, 2000. Fischer, Claude. "Studying Technology and Social Life," in Manuel Castells (ed.).High Technology, Space, and Society. Sage, Beverly Hills, Calif, 1985. Fouts, Roger. Next of Kin. William Morrow, New York, 1997. Galbraith.John Kenneth. The Anatomy of Power. Hamish Hamilton, London, 1984. Gardner, Gary, and Payal Sampat. "Mind over Matter: Recasting the Role of Materials in Our Lives." Worldwatch Paper 144, Worldwatch Institute, Washington, D.C., 1998. Gelbart, William. "Data bases in Genomic Research." Science, 23 October 1998 ...»

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