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DNA Content Analysis and Invitro Cytotoxicity in Bladder Cancer Patients. Flow Cytometric Evaluation of DNA Content Analysis and Therapeutic Responses (Invitro Cytotoxicity) of Transitional Cell Carcinoma Patients

Автор:   Saurabh V.
мягкая обложка
80 страниц
2010 год
Издательство:   Книга по Требованию
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1891 руб
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Описание:DNA analysis is the second most important application of flow cytometry. Tumor DNA content has been found to be of prognostic importance for patients with many malignant tumors, such as carcinoma of urinary bladder. Transitional Cell Carcinoma accounts for more than 90% of bladder cancer. Grade II samples were 70%. In-vitro cytotoxic studies on patients own cultured tumor tissue cells was done for the optimum cytotoxic combination of chemotherapeutic drugs and immunomodulators. This study was proposed investigate the DNA content evaluation and invitro monitoring of cytotoxicity produced by various chemotherapeutic agents viz Doxorubicin, Mitomycin C and Cisplatin on cultured cancer cells of patients having superficial TCC, individually as well as in combination of immunomodulating agents BCG and interferon alpha- 2b. Out of 25 samples nine were were aneuploid, one diploid and one tetraploid. Out of 22 samples on which cytotoxicity test performed, 36. 4% showed maximum cytotoxicity to the combination therapy and 5 showed to BCG drug. DNA content of individual cells give information about their ploidy in tumours and the distribution of cells across the cell cycle.


Orthomol Immun Junior Сила иммунитета на страже Вашего ребенка Orthomol Immun Junior Сила иммунитета на страже Вашего ребенка

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The Language of the Genes

This gets shorter with age. In a baby it is about twenty thousand letters long, while in a sixty year-old it is less than half that length. Cells from tumours have lost even more DNA from the chromosome ends. About forty letters are dropped from this section of the message each time a cell divides, so that an old body works from an imperfect instruction manual, full of typographic errors. The same happens to mitochondria! genes, which are shot full of holes as rhe years conrinue their inexorable progress. Old age is itself in part the result of genetic accidents. Human cells in culture age more quickly when they carry a defect which increases the mutation rate and some children who inherit a tendency towards cancer also show symptoms of senility much earlier than normal. The immune system, which has the highest mutation rate of any part of the body, often fails as the years pass by. It seems that the decay of our elderly selves is, to some degree, a consequence of mutation. The influence of old age in damaging sperm and egg adds a certain irony to the claims of one institution devoted to the reversal of the decay of the human race, the Centre for Germinal Choice in California, in which Nobel Prize-winners make genetic deposits for hopeful mothers ...»

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